RAPID MICROPROPAGATION OF VU NU ORCHID (ONCIDIUM SP.) BY USING TISSUE CULTURE TECHNIQUE

Tran Van Minh; Nguyen Van Khoa; Bui Thanh Hoa

doi:10.12955/cbup.v5.1103

Tran Van Minh International University, Vietnam National University Ho Chi Minh City, Viet Nam
Nguyen Van Khoa International University, Vietnam National University Ho Chi Minh City, Viet Nam
Bui Thanh Hoa International University, Vietnam National University Ho Chi Minh City, Viet Nam

DOI: https://doi.org/10.12955/cbup.v5.1103

Keywords: Oncidium, elite, callus, protocorm like bodies (PLBs), micropropagation

Abstract

: The high demand of Oncidium orchids leads us to find out efficient methods of propagating them. However, the propagation rate of traditional methods are low in nature and a hybrid seed is not genetically stable. Thus, plant cell biotechnology is examined as the most effective way to resolve the barrier of elite clone production. Shoot clusters were cultured on MS medium supplemented with 2,4-D (1 mg/l) for callus induction (76.19%) before induced callus was favoured for PLBs regeneration (98 PLBs/callus cluster) on MS medium supplemented with NAA (0.75 mg/l); the combination of BA (0.5 mg/l) and NAA (0.5 mg/l) was favoured for PLBs regeneration (28.18 PLBs/shoot cluster) from shoots cultivation. The PLBs (79.21 PLBs/PLB cluster) were then proliferated on MS medium supplemented with NAA (1 mg/l) and BA (1 mg/l) for shoot regeneration (12.42 shoots/PLBs cluster). Multiple-shoots were divided to 3-4 shoots/cluster for micropropagation on the MS medium supplemented with the combination of BA (0.25 mg/l) and NAA (0.25 mg/l) to reach 11.66 shoots/cluster. Shoots were finally separated to single-shoot for rooting on the MS medium supplemented with NAA (0.75 mg/l). A scheme for Oncidium micropropagation using PLBs culture techniques was set up.

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