Mitochondrial Defects And Their Role In Development Of Prostate Cancer
Abstract
Introduction and Objectives: One of the characteristic changes of tumor formation is accumulation of genetic disorders in mitochondrial and nuclear genome. Mitochondrial disorders, from its side, are responsible for failure of metabolism, apoptosis, cell growth, formation of reactive oxygen species, etc. Overprpoduction of reactive oxygen species (ROS) significantly impacts the respiration chain enzymes and entirely the antioxidant system of mitochondria. Finally this may become a favorable condition for normal cells transformation.
The purpose of the presented work was to study the mitochondrial defects and to establish their role in prostate cancer development.
Results: Experimental results demonstrate significant increase of the activity of mitochondrial succinate dehydrogenaze (complex II) of the malignant epithelial cells of prostate, and slight changes in cytochrome oxydase (complex IV) activity. Also significant activation of the antioxidant system (glutathione-dependant system) of mitochondria in prostate malignant epithelial cells was revealed.
Conclusion: The above mentioned mitochondrial changes (II and IV complexes of respiration chain, activity of the antioxidant system) partially demonstrate the alterations in mitochondrial energy metabolism, which from its side, may indicate to resistance of prostate cancer cells and correspondingly to intensification of proliferation processes.
References
Alejnikova T.L.//Biochemistry the Management to a practical training//1988.
Balendiran G.K., et al. //The role of glutathione in cancer// J. Cell Biochem. Funct., vol. 22. 2004: 43-52.
Brusque A. M. et al// Inhibition of the mitochondrial respiratory chain complex activities in rat cerebral cortex by methylmalonic acid// Neurochemistry International vol.7. 2002 :593-601.
Chen J.Z., Gokden N., Greene G.F., Green B., Kadlubar F.F.,// Simultaneous generation of multiple mitochondrial DNA mutations in human prostate tumors suggests mitochondrial hyper-mutagenesis //J. Carcinogenesis. vol.24. 2003 :1481–87.
Dakubo GD, et al.// Altered metabolism and mitochondrial genome in prostate cancer// J Clin Pathol. Jan 59(1), 2006 :6-10.
Daniel G Clark //Reference guide to the Basic Science Aspects of the Mitochondria. Section III – Mitochondrial Function in Oxidative Phosphorilation// ©American Medical Research, LLC, Sarasota, Florida, 2000 : 1-48.
Della Rovere F., et al.//SH grups and glutathione in cancer patients//J. Anticancer Res., vol.20, 2000:1595-98.
Higuchi, M et al.// Mitochondrial DNA determines androgen dependence in prostate cancer cell lines// Oncogene. Vol.25. 2006 :1437–1445.
Kanbagli Öznur et al.// Hepatic Mitochondrial Prooxidant and Antioxidant Status in Ethanol-Induced Liver Injury in Rats// B.P.B., No11. 2002:1482-1489.
Khandrika Lakshmipathi , Kumar Binod, Koul Sweaty et al // Oxidative Stress in Prostate cancer // Cancer Letters vol.282. 2009 :125–136.
Lim S.D., Sun C., Lambeth J., D. et al // “Increased Nox1 and hydrogen peroxide in prostate cancer,” // J.Prostate, vol.62. 2005 : 200–07.
Martínez Federico et al.// Structural and Functional Changes in Mitochondria Associated with Trophoblast Differentiation: Methods to Isolate Enriched Preparations of Syncytiotrophoblast Mitochondria// Endocrinology, v. 138, No.5. 1997:2172-2183.
McNeal, J E; Redwine, E A; Freiha, F S. et al Zonal distribution of prostatic adenocarcinoma. Correlation with histologic pattern and direction of spread. Am J Surg Pathol. vol.12. 1988 :897–906.
Nijtmans L.G.J., Smeitink J.A.M. Mitochondrial Medicine // Ned Tijdschr Klin Chem Labgeneesk. vol.32, #3. 2007: 188-191.
Petros J.A., Baumann A.K., Ruiz-Pesini E., Amin M.B., Sun C.Q., Hall J., Lim S., Issa M.M., Flanders W.D., Hosseini S.H., Marshall F.F., Wallace D.C. mtDNA mutations increase tumorigenicity in prostate cancer, Proc. Natl. Acad. Sci. (USA) vol.102. 2005 : 719–24.
Ripple, M.O., et al. // Prooxidant-antioxidant shift induced by androgen treatment of human prostate carcinoma cells // J. Antioxidant and Redox Signaling, vol. 1. 1999: 71-81.
Singh Keshav K, Desouki Mohamed M, Franklin Renty B, Costello Leslie C// Mitochondrial aconitase and citrate metabolism in malignant and nonmalignant human prostate tissues // Molecular Cancer vol. 5. 2006 :14 -17.
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