Potential Chemopreventive Effect Of “Procont”On Mirna Expression In CBA/CA Mice

Mónika Szirmai, Krisztina Juhász, András Bertha, Katalin Gombos, Péter Révész, István Ember

Abstract


Background: There is emerging molecular evidence concerning themodification of miR expression pattern after the treatment with chemopreventive agents. We investigated whether a potential chemopreventive agent called “Procont” deriving from a biological systemhas a positive effect on the DMBA induced dysregulation of let-7a, miR-21 and miR-146amiRNAs.Materials and Methods: CBA/CA H2k inbred micewere fed by Procont for 7 days before the intraperitonealinjection of DMBA. After one weekof the beginning of Procont diet weanalized the let7a, miR-21, miR-146agene expression in vital organs of mice. Results:The Procont feeding significantly decreased all of the investigated miRNA expression compared with either the mice on a normal diet in the control group or mice were exposed to DMBA alone.Conclusion:The results indicate that“Procont” diet has a potential chemopreventiveeffect in mice exposed to DMBA.

Keywords


Procont,Mirna expression,Chemoprevention

Full Text:

PDF

References


Bartel DP: MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004; 116(2):281-97.

Zhang B, Pan X, Cobb GP, Anderson TA: microRNAs as oncogenes and tumor suppressors. Developmental Biology. 2007; 302(1):1-12.

Croce CM: Causes and consequences of microRNA dysregulation in cancer. Nat Reviews Genetics. 2009; 10(10):704-14.

17 Zhu S, Wu F, Nie D, Sheng S, Mo YY: Micro-RNA-21 targets tumor suppressor genes in invasion and metastases. Cell Research.2008; 18: 350-9.

Pasquinelli, A., Reinhart, B., Slack, F., Maller, B., and Ruvkun, G. (2000): Conservation across animal phylogeny of the sequence and accession numbers AY941100 and AY941101 temporal regulation of the 21 nucleotide C. eleganslet-7 heterochronic regulatory RNA. Nature. 2000;408: 86–89.

Akao Y, Nakagawa Y, Naoe T: let-7 microRNA functions as a potential growth suppressor in human colon cancer cells. Biol Pharm Bull. 2006; 29(5):903-6.

Steven M. Johnson, HelgeGrosshans, Jaclyn Shingara, Mike Byrom, Rich Jarvis, Angie Chang, Emmanuel Labourier, Kristy L. Reinert, David Brown, Frank J. Slack: RAS is regulated by the let-7 micrRNA family. Cell.2005;120:635-647.

Zhao JL, Rao DS, Boldin MP, Taganov KD, O'Connell RM, Baltimore D: NF-{kappa}B dysregulation in microRNA-146a-deficient mice drives the development of myeloid malignancies. ProcNatlAcadSci U S A. 2011; 108(22):9184-9.

Naugler WE, Karin M: NF-kappaB and cancer-identifying targets and mechanisms. CurrOpin Genet Dev. 2008; 18(1):19-26.

Alberto Izzotti, George A. Calin,PatrizioArrigo,Vernon E. Steele, Carlo M. Croce, Silvio De Flora: Downregulation of microRNA expression in the lungs of rats exposed to cigarette smoke. FASEB J. 2009; 23(3): 806–812.

Pogribny IP, Tryndyak VP, Boyko A, Rodriguez-Juarez R, Beland FA, Kovalchuk O: Induction of microRNAome deregulation in rat liver by long-term tamoxifen exposure. Mutation Research. 2007; 619(1-2):30-7.

Zhang B, Pan X: RDX induces aberrant expression of microRNAs in mouse brain and liver. Environ Health Perspect. 2009; 117(2):231-40.

KrisztinaJuhász, KatalinGombos, MónikaSzirmai, PéterRévész, Ingrid Magda, KatalinGőcze, István Ember: DMBA Induces Deregulation of miRNA Expression of let-7, miR-21 and miR-146a in CBA/CA Mice. In vivo.2012; 26(1):113-7.

IstvánSzanyi, LászlóLujber, ImreGerlinger, JózsefPytel, Miklós Bauer, AndrásCsejtey, EszterSzele, KatalinGombos, István Kiss, Sergey Seredenin, MiladaYarkova, István Ember: In vivo effects of Afobazole (2-Mercaptobenzimidazole Derivative) on the 7,12-Dimethylbenz(a)anthracene-induced oncogene and suppressor gene expression. In vivo.2007; 21:1059-1064.

FerencBudán, IstvánSzabó, TímeaVarjas, GhodratollahNowrasteh, TamásDávid, PéterGergely, ZsuzsaVarga, KornéliaMolnár, BalázsKádár, ZsuzsaOrsós, István Kiss, István Ember: Mixtures of Uncaria and Tabebuia extracts are potentially chemopreventive in CBA/CA mice: a long term experiment. Phytotherapy Research. 2011; 25:493-500.

Izzotti A, Calin GA, Steele VE, Cartiglia C, Longobardi M, Croce CM, De Flora S: Chemoprevention of cigarette smoke-induced alterations of MicroRNA expression in rat lungs. Cancer Prev Res. 2010; 3(1):62-72.

TameneMelkamu, Xiaoxiaou Zhang, Jiankang Tan, Yan Zeng, FekaduKassie: Alteration of miRNA expression in vinyl carbamate-induced mouse lung tumors and modulationbychemopreventive agent indole-3-carbinol. Carcinogenesis. 2010;31(2):252-258.

Zhu S, Wu F, Nie D, Sheng S, Mo YY: Micro-RNA-21 targets tumor suppressor genes in invasion and metastases. Cell Research.2008; 18: 350-9.




DOI: http://dx.doi.org/10.12955/emhpj.v3i0.350

Refbacks

  • There are currently no refbacks.


Print ISSN 1804-5804, Online ISSN 1804-9702

(c) 2018 CBU,o.p.s.